APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Target Retrieval Solution buffer.
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Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 2% BSA
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
33 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
high mobility group box 1,HMG-1,HMG1,HMG3,SBP-1
Cellular Localization
Nucleus,Cytoplasm,Secreted,Cell membrane,Endosome
Background
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX639941 IHC-P Image
IHC-P analysis of human placenta (amnion and chorion) tissue using GTX639941 HMGB1 antibody [HMV317] HistoMAX™. Strong nuclear HMGB1 staining of chorion cells and moderate nuclear HMGB1 staining of amnion cells.
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GTX639941 IHC-P Image
IHC-P analysis of human esophageal squamous cell carcinoma (ESCC) tissue using GTX639941 HMGB1 antibody [HMV317] HistoMAX™. Squamous cell carcinoma with strong HMGB1 staining of tumor cells.
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GTX639941 IHC-P Image
IHC-P analysis of human primitive neuroektodermal tumor (PNET) from soft tissue using GTX639941 HMGB1 antibody [HMV317] HistoMAX™. Primitive neuroektodermal tumor (PNET) showing intense HMGB1 staining of tumor cells.
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REFERENCE
There are currently no references for HMGB1 antibody [HMV317] HistoMAX™ (GTX639941). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HMGB1 antibody [HMV317] HistoMAX™ (GTX639941). Be the first to share your experience with this product.